| THE BULLETIN OF TOKYO DENTAL COLLEGE | |
| Vol. 48 No. 4 November 2008 | |
| ISSN: 0040-8891 UBIC: 66 | |
Abstract |
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| The effects of X-ray and carbon ion irradiation on DNA and genes in head and neck carcinoma cells
were examined. Four head and neck cancer cell lines (squamous cell carcinoma, salivary gland cancer, malignant melanoma,
normal keratinocyte) were treated with 1, 4, and 7 GyE of carbon ion, or 1, 4, and 8 Gy of X-ray, respectively. DNA and
RNA in the treated cells were extracted and purified. PCR-LOH (polymerase chain reaction-loss of heterozygosity) analysis
with 6 microsatellite regions on chromosome 17 was performed to determine DNA structural damage, and then microarray
analysis was performed to reveal changes in gene expression. PCR-LOH analysis detected high LOH in cells treated by
radiation, indicating that most of the damage by X-ray occurred in the target region on one of the homologous chromosomes.
However; carbon ion caused homo- deletion, which means deletion of the counterparts in both homologous chromosomes.
Key words: Head and neck carcinoma-loss of heterozygosity (LOH) - X-ray irradiation -Carbon ion irradiation. |
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