| The Japanese Dental Science Review | |
| Vol. 45 No. 2 2009 | |
| ISSN: 1882-7616 UBIC: 99 | |
| SUMMARY | |
| ICleft lip with or without cleft palate is the most common congenital anomaly in craniofacial complex, which is our target to deepen our understanding
toward a molecular mechanism of dento-craniofacial morphogenesis. The hypothesis that the maternal effects in addition to the genetic ones play important roles on
the dento-craniofacial morphogenesis in mice has been tested based on each developmental stage. Firstly, the maternal effects on the intrauterine craniofacial development
in the mouse fetus were examined by means of embryo transfer technique, skeletal staining and cephalometry, indicating that the maternal effects were one of important
factors on the intrauterine craniofacial morphogenesis of CL/Fr mice. Secondly, when the molecular nature of the maternal effects is elucidated, maternally derived growth
factors may p[ay important roles on mouse fetus development. Bone morphogenetic protein 4 (BMP4) and epidermal growth factor (EGF) function antagonistically, yet
are coupled in the regulation of initial chondrogenesis. Smad 1 serves as a point of convergence for the integration of two different growth factor signaling pathways
during chondrogenesis in the mouse fetal mandible. Lastly, transforming growth factor-beta3 (TGF-β3) promoted fusion of cleft lip in the mouse fetus through the
molecular pathways. In fact, microsurgical repair of cleft lip in the fetus that produced scarless fusion is mediated by TGF-β3 regulation of mesenchymal cell proliferation
and migration at the site of repair. In addition, TGF-β3 promoted cell proliferation and angiogenesis in lip mesenchymal tissues. These events lead to enhancement
of the lip fusion in the presence of TGF-β3. In neonatal mouse, application of exogenousTGF-β3 to decrease type I collagen accumulation and consequential scar
formation provide the opportunities for the clinical augmentation of scar reduction after cleft lip repair. These findings indicate that the environmental factors provided
by the dams cannot be ignored in the etiology of a craniofacial anomaly and/or development in the mouse model in addition to genetic ones.
KEYWORDS: Prenatal maternal effect; Cleft lip and palate; Embryo transfer, in vitro organ culture; Fetal surgery; Growth factor; Transcriptional factor. |
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