INTERNATIONAL JOURNAL OF ORAL-MEDICAL SCIENCES

Vol. 7 No. 2      December - 2008

ISSN: 1347-9733      UBIC: 136-M

Abstract

The purpose of this study was to identify and to characterize of Malassezia spp. in the oral cavity of dog. One hundred and seventeen strains of Malassezia spp. were isolated from fresh clinical isolates of fungi from 20 dogs. The isolates grew well on Sabouraud agar, and produced catalase and precipitates on Tween-medium, and hydrolyzed esculin. Colony morphologies and sizes were characteristic on CHROMagar Malassezia (CHROM): they developed small, violet color, medium, violet color with pink edge, or large, pale pink color colonies. Molecular investigations of 18 isolates in three different morphological colonies on CHROM from 6 subjects were carried out by chitin synthase 2 (CHS2) gene sequence analysis. The results showed that isolates constitute three genetic types (Ac, Bc, and Cc). Type Ac, Bc, and Cc consisted of 3, 8, and 7 isolates, respectively. The three isolates from only one subject were grouped into the same genotype. These results suggest that the isolates in the oral cavity of dog were Malassezia pachydermatis, and the isolates in CHS2 genetic group were diverse. The relationship between colonial morphology and genetic typing was inconsistent. Keywords: animal, fungus, Malassezia, dog.

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