JOURNAL OF OSAKA DENTAL UNIVERSITY

Vol. 42 No. 1      APRIL - 2008

ISSN: 0475-2058      UBIC: 172

SUMMARY

Recent studies have indicated that bacteria possessing biofilm phenotype cause persistent infections. However, the exact distribution of biofilm-forming bacteria in periodontitis lesions remains poorly understood because of difficulties with isolation procedures and because repetitive subcultures can disrupt the biofilm organization. We isolated and identified viscous material-producing bacteria with biofilm-like structures from subgingival plaque in periodontal pockets. Samples were collected from four patients al Osaka Dental University Hospital, and incubated anaerobically for 48 hrs on blood agar plate. The isolates achieved were transferred into enriched-trypticase soy broth for measurement of viscosity. Cell-surface structures were observed by scanning electron microscopy. Forty bacterial strains having viscosity and meshwork-like structures were isolated. identification of these isolates revealed three anaerobic rods (Eubacterium sp., Fusobacterium sp. and Prevotella sp.), one anaerobic coccus (Veillonella spp.), three facultative anaerobic rods (Actinomyces spp., Capnocytophaga sp. and Eikenella sp.), one facultative anaerobic coccus (Streptococcus spp.), and one aerobic/microaerophilic rod (Kingella sp.). The results indicate that a far larger number than expected of bacterial genera in periodontal pockets have the ability to produce viscous materials and form biofilm-like structures. This may suggest that this screening method is useful for grasping the distribution of biofilm-forming bacteria in a periodontal pocket. (J Osaka Dent Univ 2008; 42: 1-7) Key words: Oral bacteria; Biofilm; Periodontitis; Periodontal abscess.

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