THE JOURNAL OF THE TOKYO DENTAL COLLEGE SOCIETY

Vol. 108 No. 2      April 2008

ISSN: 0037-3710      UBIC: 342-J

ABSTRACT

Telomeres of mammalian chromosomes are composed of long tandem repeats TTAGGG which bind in a sequence-specific manner two proteins-telomere-repeat-binding factor. TRF1 and TRF2. Tankyrase was a poly(ADP-ribose)-polymerase that is initially identified through its interaction with TRE. In this study, we analyzed the subcellular localization of TRF2 and tankyrase in living osteoblast-like MC3T3-E1 cells by using fusion proteins with different spectral variants of green fluorescent protein (CF Two fluorescent proteins, cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP)were used as markers to visualize TRF2 and tankyrase. respectively. Osteoblast- like MC3T3-E1 cells co-transfected with TRF2-CFP and tankyrase-YFP showed that TRF2- CFP appeared to be concentrated at distinct subcellular structures(presumably the telomeres). whereas tankyrase-YFP was uniformly distributed throughout the cytoplasm. (The Shikwa Gakuho, 108 : 117-121, 2008) Key words: Telomere-repeat-binding factor Tankyrase, Green fluorescent protein, Aging Short running head TRF and tankyrase in bone cells.

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